Fig. 4
From: IκBα mediates prostate cancer cell death induced by combinatorial targeting of the androgen receptor
Quantitative analysis of androgen regulated genes altered by combinatorial AR targeting. a Quantitative real-time PCR analysis of an independent RNA sample set, generated by treatment of LNCaP cells with vehicle control [VEH], 1 μM vorinostat [VOR], 2.5 μM bicalutamide [BIC] or the combination of 1 μM vorinostat and 2.5 μM bicalutamide [BIC + VOR] in triplicate for 6 h. The expression of KLK2, KLK3, NKX3-1, IGF1R, NFKBIA and C1orf116 was normalised to GUSB and HPRT1. Fold changes are expressed relative to vehicle control. Values indicated are the mean of technical and biological replicates ± SEM, and are representative of three independent experiments. * = p < 0.05 using one-way ANOVA with Bonferroni post-hoc test, compared with vehicle control. # = p < 0.05 using t-test compared with vorinostat, and ^ = p < 0.05 using t-test compared with bicalutamide. b Western blot analysis of lysates from LNCaP cells cultured with either vehicle control [VEH], 1 μM vorinostat [VOR], 2.5 μM bicalutamide [BIC] or the combination of 1 μM vorinostat and 2.5 μM bicalutamide [BIC+VOR] for 12, 48, or 96 h. Steady state levels of PSA (KLK3) are shown, and hsp90 is shown as a loading control