Fig. 2
From: Tumor slice culture system to assess drug response of primary breast cancer
Assessment of proliferation in tumor slices by EdU incorporation. a Co-staining for EdU (red), Cytokeratin (green) and DAPI (blue). Scale bar indicates 100 μm. b Screenshots of semi-automated measurement of Cytokeratin area and number of EdU positive cells using image analysis software. c Example of tumor proliferation after prolonged culture of tumor slices. Multiple image fields were analyzed per tumor slice. Heterogeneity in proliferation is visualized in this graphical representation by interquartile ranges. Each black dot represents one image field. Red bars indicate interquartile range and blue bars represent median values. d Proliferation rate of multiple tumors after incubation for up to seven days. Maximum incubation times varied per tumor depending on availability of tumor slices. Black dots indicate median values and error bars represent interquartile range