Fig. 3
From: Induction of proto-oncogene BRF2 in breast cancer cells by the dietary soybean isoflavone daidzein
Daidzein induces U6 snRNA and tRNAi Met specifically in MCF-7 cells without inducing proliferation. MCF-7 (a) and MDA-MB-231 (b) cells were treated with 0, 3, 10 μM daidzein for 48 h. U6 snRNA and tRNAi Met expression was then analysed by qRT-PCRusing the ΔΔCt method with RPS13 expression levels as a reference for normalization. Meta-analysis of three independent experiments performed in triplicate was completed using one-way ANOVA with a Tukey’s post-test with a 95 % confidence interval (Graphpad Prism 3.03); * = p <0.05; ** = p < 0.01; *** = p < 0.001. CellTiter-Glo® (Promega) was used to count MCF- 7 (c) and MDA-MB-231 cells (d) after 24, 48 and 72 h treatment with 0, 3 or 10 μM daidzein, as indicated. Each dose and time point was performed in triplicate. MDA-MD-231 cell proliferation significantly decreased with 10 μM daidzein after 48 h (p < 0.05) and 72 h (p < 0.01) treatment. MCF-7 cell proliferation significantly decreased at 48 h (p < 0.01) with 3 μM daidzein treatment. At 10 μM daidzein treatment cell proliferation was significantly inhibited in MCF-7 cells at 24 h (p < 0.05), 48 h (p < 0.01) and 72 h (p < 0.01)