Fig. 4

Microarray quadrant analysis for differentially expressed genes in parental versus resistant SINE compound-treated cells. Untreated parental cells were compared to treated parental cells (x-axis) while untreated resistant cells were compared to treated resistant cells (y-axis) and genes that were 1.5 fold up- and down- regulated in each of the SINE compound treated cell lines were plotted. a Fold change in mRNA expression following KPT-185 treatment of apoptosis and autophagy-related genes. NGFR induction was similar in both cell lines, while TP53INP1 and BIRC3 induction was stronger in parental cells; EGR-1 was induced exclusively in resistant cells while IL1B was down-regulated only in parental cells. b Fold change in mRNA expression following KPT-185 treatment of proliferation-related genes. EDN-1 induction was stronger in resistant cells; BTG2 induction was stronger in parental cells; KITLG was exclusively down-regulated in resistant cells. c Fold change in mRNA expression following KPT-185 treatment of cell-cycle and cytoskeleton-related genes. PCLO induction was stronger in parental cells; CCNG2 induction was stronger in resistant cells; TOB1 induction was stronger in parental cells; CCNE2 and INHBE were exclusively down-regulated in resistant and parental cells, respectively. Black line represents perfect correlation as reference