Fig. 3
Features of cancer selectivity of human oncolytic adenoviruses on murine cells. (a) Cells were seeded in 60-mm dishes at a density of 106 for 24 h and then collected. The cell lysates were immunoblotted for cyclin E protein and actin. Actin was used as a loading control. (b) Cells were mock-infected or infected with AdGFP, Adwt, dl1520, or Ad-cycE at 3.5 MOI (for A549 cells) or 10 MOI (for ED-1 and NIH/3T3 cells). Cytopathic effect (CPE) was observed at 72 h p.i. and photographed with an inverted microscope Olympus CKX41. The cell viability percentage was determined, and the values represent the means ± S.D. of triplicate samples compared with the mock-infected group. (c) ED-1 or NIH/3T3 cells were infected with Adwt, dl1520, and Ad-cycE at 10 MOI for 18 h or 120 h. The virus yields were determined by infection unit method and expressed as burst ratios, representing virus yields at 120 h p.i. relative to virus yields at 18 h p.i. The values represent the means ± S.D. of triplicate samples