Evaluation of an assay for methylated BCAT1 and IKZF1 in plasma for detection of colorectal neoplasia

BMC Cancer

Table 4 Blood methylation levels in 12 CRC cases positive before and after tumour resection

Case characteristics		Proportion of methylated BCAT1 and IKZF1¹ (% of total yield)
Tumour location	Stage	Before resection	After resection	Δ Days
Sigmoid	l	<0.00001	0	140
Splenic flexure	l	<0.00001	0	48
Caecum	llA	1.8	0	115
Ascending	llA	0.7	<0.00001	70
Sigmoid	llA	5.6	0	162
Sigmoid	llA	2.6	0	64
Ascending	llA	1.7	0	83
Ascending	llA	1.4	0	39
Caecum	lllA	5.0	0	58
Rectum	lllA	0.8	0	47
Ascending	lllB	0.9	0	50
Sigmoid	lllC	0.6	0.6	157

¹The lower limit of the linear range for the qPCR assay was 100pg per reaction. The average DNA amount per reaction was 2 ng, thus methylation levels estimated to be <5% but above zero are extrapolated and most likely inaccurate

ISSN: 1471-2407