Fig. 4

DCIS cells migrate preferentially towards CAFs in MAME co-culture. Representative DIC/fluorescent overlay image of MCF10.DCIS cells (unlabeled) with CAF40TKi fibroblasts (green) at day 1 (a) and 3 (b). Scale bar, 50 μm. Orange arrow indicates migration direction (ref. Additional file 9: Video S2). c Video snapshot of live cell imaging between days 3 and 4 shows a tumor spheroid attached to a single fibroblast (Arrow; ref. Additional file 9: Video S2). d At day 7, tumor cells have proliferated and formed protruding structures, which are connected to fibroblasts. Scale bar, 100 μm. e Representative 3D reconstructed image showing aerial view (e) and side view (f) of MAME MCF10.DCIS:CAF40TKi co-culture (ref. Additional file 11: Video S3). A primary MCF10.DCIS spheroid (dashed white circle) can be seen with lateral protrusions towards fibroblasts (CFSE-labeled and pseudo-colored white). Nuclei are stained with DAPI (purple). 1 Unit = 45 μm