Fig. 1
a Representative histogram of FACS analysis of H2B-GFP label retention in RPMI8226-Tet-H2B-GFP cells 0 and 3 days after a 24-h pulse with Bz (4 nM and 8 nM). Percentages = Percent of H2B-GFPHIGH ± standard deviation. b Quantification plot of H2B-GFPHIGH label retention. ****, P < 0.0001 comparing surviving day 3 DMSO to 4 nM Bz or 8 nM Bz, (unpaired t test). c Representative histogram of FACS analysis of H2B-GFP label retention 6 days after 24-h pulse with Bz. Percentage of cells was calculated using BD FACSDiva software (BD). d Western blots for p21CIP1 protein in nuclear extracts of cells surviving proteasome inhibition 0, 3 and 6 days after drug washout. Total Histone H3 was used as a loading control. e & f Detection and quantification of p21CIP1 in H2B-GFPHIGH label-retaining cells at 3 and 7 days after drug washout by IF. Quantification was done using ImageJ. * p = 0.0361 comparing day 3 H2B-GFP-positive cells in DMSO vs. 4 nM Bz (unpaired t test). * p = 0.0475 comparing day 7 H2B-GFP-positive cells in DMSO vs. 4 nM Bz (unpaired t test). g Western blots for CDK6 protein in cells surviving proteasome inhibition 3 days after drug washout. GAPDH was used as a loading control. Scale bar =20 μm