Figure 4

Coculture with HMF induces invasive transition in the DCIS model. A) Schematic image of coculture experiments with the DCIS model in the center chamber of a triple microchannel and HMFs in the side chambers. B) Bright-field images of mammary duct, mammary duct filled with MCF10a cells, and mammary duct filled with DCIS cells either alone or in coculture with HMFs. Scale bars represent 100 μm (main chart) and 50 μm (close up image at the right). The average number (C) and area (D) of invasive lesions in the different culture conditions were quantified across three independent experiments with at least three channels per experiment. A student Student’s t-test was used to determine significant differences between culture conditions (p < 0.05 is considered to be significant).