Figure 2

Mammary duct model with 3D lumen structure. A) Volume-rendered confocal image of MCF10a cells lining a lumen patterned through a collagen I hydrogel and coated with Matrigel. Cells were cultured for 72 hours and then stained for e-cadherin (red) and nuclei (blue). Scale bar represents 50 μm. B) Confocal slice of bottom of the lumen to show e-cadherin localization around cell junctions. Scale bar represents 5 μm. C) Volume-rendered confocal image of MCF10a cells lining a lumen and stained for laminin V (green), nuclei (blue) and GM130 (red) to demonstrate cell polarization. Scale bar represents 1 μm.