Figure 5

Activation of NF-κB in murine lung and intestinal tissues. (A) The activation of NF-κB was detected by EMSA in intestinal tissues. Representative figures are shown for (a) unirradiated control mice, (b) irradiated mice 24 h after exposure to 20 Gy, (c) flutamide-treated mice 24 h after exposure to 20 Gy and (d) castrated mice 24 h after exposure to 20 Gy. These findings demonstrate that there was significant attenuation of RT-induced the binding activity of nuclear NF-κB in castrated mice, whereas androgen deprivation by flutamide did not induce NF-κB activation. (Y axis represents the relative level which is normalized by the level of NF-κB activity in control condition). (B) Expression of COX-2 protein in irradiated murine intestinal tissue with or without androgen deprivation including castration and flutamide administration. (a, control; b, irradiation; c, castration alone; d, castration plus irradiation; e, flutamide administration; f, irradiation plus flutamide administration). Triplicate experiments were performed for the analysis. (Y axis represents the relative protein level which is normalized by the protein level of COX-2 in control condition). (C) Expression of β-catenin, vimentin and p-Akt in the intestinal tissues after various treatments (a, control; b, treated with TGF-β1; c, treated with TGF-β1+ wortmannin; d, irradiated tissue 24 h after 20 Gy; e, after pretreatment with wortmannin for 12 h followed by 20 Gy irradiation). Triplicate experiments were performed for the analysis.