The high affinity selectin glycan ligand C2-O-sLex and mRNA transcripts of the core 2 β-1,6-N-acetylglusaminyltransferase (C2GnT1) gene are highly expressed in human colorectal adenocarcinomas

Table 3 C2-O-sLe^x was compared to sLe^x expression in primary colorectal adenocarcinomas and metastatic liver tumors.

	Differentiation Status
	Well differentiated		Moderately differentiated		Poorly differentiated
Antibody staining reactivity in colorectal adenocarcinomas	0+	(NE)	0+	(1+)*	NE	(NE)
	1+	(2+)*	1+	(1+)	1+	(3+)*
	2+	(2+)	2+	(3+)*	2+	(3+)*
	3+	(3+)	3+	(3+)	3+	(3+)
Antibody staining reactivity in metastatic liver tumors	NE	(NE)	0+	(0+)	NE	(NE)
	NE	(NE)	1+	(2+)*	NE	(NE)
	2+	(2+)	2+	(2+)	2+	(3+)*
	3+	(2+)	3+	(3+)	3+	(3+)

Serial sections of individual tissues were stained with CHO-131 mAb that detects C2-O-sLe^x and CSLEX1 mAb that detects sLe^x. The numbers in the body of the table indicate the intensity of reactivity of a representative single tissue with CHO-131 mAb. The bracketed numbers indicate the intensity of reactivity of a serial section of the same tissue with CSLEX1 mAb. NE = not examined. * indicates a difference in reactivity between CHO-131 and CSLEX1 mAbs.

ISSN: 1471-2407