Figure 2

MIF targeting by antisense transfection results in reduced proliferation and restoration of contact inhibition. The generated LN18 MIF antisense-expressing clones (as1 + as2) and controls (wildtype wt, control - empty vector-transfected - clone c1) were plated on 96-well plates at the same cell number. Growth was measured the following days by amido-black assay. At day 8 the antisense clones as1 and as2 reached 48% (p 0.0043) and 57% (p 0.0007), respectively of the cell density of wildtype cells (A). DNA synthesis was significantly (* p < 0.005) compromised in clones at higher cell densities (B).