Figure 4

Effects of treatment with IFN-γ or IL-4 on Panc-1 and SU86.86 pancreatic cancer cells. Cells were treated with either 2000 IU/ml of recombinant IFN-γ or 40 ng/ml of recombinant IL-4 for 24 or 48 hours and analyzed by FACS. (B) IFN-γ treatment resulted in a marked increase of B7-H3 expression on SU86.86 cells after 24 hours (green line) and 48 hours (red line). (D) IL-4 treatment resulted in a marked increase of B7-H3 expression on SU86.86 cells after 48 hours (red line), but not after 24 hours (green line). In contrast, (A) IFN-γ treatment led to a slight decrease of B7-H3 expression on Panc-1 cells 24 hours after stimulation (green line) with normalization 48 hours after stimulation (red line). (C) IL-4 treatment led to a slight decrease of B7-H3 expression on Panc-1 cells 24 hours after stimulation (green line) and 48 hours after stimulation (red line). The blue lines display B7-H3 expression without stimulation by IFN-γ and IL-4. The grey lines display the (isotope) control group.