Figure 2

The sensitivity of the CDKN2A and RARB SMART-MSP and MS-HRM assays. The sensitivity of the assays was tested using standard dilution series of methylated DNA into unmethylated DNA. The 100% methylated standards are indicated in red, the 10% methylated standards in blue, the 1% methylated standards in green, the 0.1% methylated standards in brown, the 0% methylated standard in orange and non template controls are indicated in black. A. The CDKN2A SMART-MSP assay (real-time amplification data). B. The CDKN2A MS-HRM assay (normalized melting curves). C. The RARB SMART-MSP assay (real-time amplification data). D. The RARB MS-HRM assay (normalized melting curves). All assays successfully detect 0.1% methylation.