Figure 4

Endocytosis proteins surround the primary cilium. Primary cilia in astrocytes were examined by IIF after co-staining with glu tubulin and AC III antibodies (red). The primary cilium was surrounded by organelles involved in endocytosis including (A) early endosomes as detected using antibodies directed to early endosome antigen 1 (EEA1), (B) late endosomes/lysosomes using antibodies directed to lysosome associated membrane protein 1 (LAMP1), (C) trans Golgi network using antibodies directed to the trans Golgi network 38 (TGN38) and (D) the Golgi complex using antibodies directed to golgin 97 (green). Nuclei were counterstained with DAPI. The inset box is an enlargement of the primary cilia in relation to the EEA1, LAMP1, TGN38 and golgin 97 staining. IIF scale bars = 15 μm.