Figure 2
From: Upstream ORF affects MYCN translation depending on exon 1b alternative splicing
Exon 1b-splicing, uAUG and MYCN translation. a) Western blot analysis of 40 μg SH-EP neuroblastoma cells transfected with either control pcDNA/GW40/lacZ (lane 1), p-MYCN (lane 2) or p-MYCN Δ1b(lane 3). Results obtained with -upper panel: a monoclonal antibody against MYCN (1:1000) and -lower panel: a monoclonal antibody against β-Actin (1:10000). b) Western blot analysis of 40 μg SH-EP cells transfected with either p-MYCN (lane 1), p-MYCN mut(lane 2), p-MYCN Δ1b(lane 3) or p-MYCN Δ1b, mut(lane 4). Results obtained with -upper panel: the anti-MYCN antibody (1:1000) and -lower panel: the anti β-Actin antibody (1:10000).