Figure 3

Expression of TEM1/endosialin by cultured endothelial cells. A. Expression of TEM1/endosialin after treatment of endothelial cells with pro-angiogenic growth factors. Cells were treated for 48 hours (see Methods) and analyzed by quantitative RT-PCR. Error bars, standard error of the mean. No significant change in TEM1/endosialin was detected. B. Induction of TEM1/endosialin after growth of endothelial cells in matrigel. Cells were grown on plastic (Control) or in matrigel (Matrigel) for 6 hours then analyzed by quantitative RT-PCR. Error bars, standard error of the mean. *p = 0.05. After 6 hours on matrigel and prior to harvest, HMVEC cells demonstrate branching tubulogenesis (lower right) while cells grown on plastic do not (lower left).