Table 4 Secondary antibodies and counterstains
Antibody | Abbreviation | Supplier | Product no. | Dilution |
|---|---|---|---|---|
Goat anti rabbit biotinylated | GARB | Dako | E0432 | 1:500 |
Goat anti mouse biotinylated | GAMB | Dako | E0433 | 1:500 |
Goat anti-mouse Alexa Fluor 488 | GAM 488 | Mol. Probes | A-11029 | 1:200 |
Avidin Alexa Fluor 488 | Avidin 488 | Mol. Probes | A-21370 | 1:200 |
Streptavidin Alexa Fluor 546 | Streptavidin 546 | Mol. Probes | S-11225 | 1:200 |
Tyramide fluorescein | Tyramide fluorescein | Perkin Elmer Life Sciences | NEL 744 | 1:50 |
To Pro | To Pro | Mol. Probes | T3605 | 1:1000 |