A proteomic strategy to identify novel serum biomarkers for liver cirrhosis and hepatocellular cancer in individuals with fatty liver disease

Table 2 The identification of the differentiating protein spots on 2D gels.

Protein Spot	Protein Identified by Mascot Search	Accession Code (Entrez Protein)	Mascot Score (Expect value in brackets)*	Sequence Coverage %**
1	ApoA1 (human)	CAA00975	247 (6.5e^-19)	80
2	ApoA1 (human)	CAA00975	236 (8.1e^-18)	75
3	Pro-ApoA1 (human)***	CAA00975	195 (1.0e^-13)	62
4	ApoA4 (human)	AAA51748	338 (5.1e^-28)	64
5	CD5L/AIM (human)	AAD01446	218 (5.1e^-16)	68

The protein spots are as numbered in Figure 1. The * Mascot score is shown as -10*Log(P), where P indicates the probability of a random match. Scores > 78 indicate that the protein identification is statistically significant (P < 0.05). ** Sequence coverage from matched peptides in spectra is provided in additional figures 1-10. *** Comparisons between zoomed mass spectra for spots 1 and 2 versus spot 3 are provided in additional figure 11. The pro-peptide sequence (RHFWQQDEPPQSPWDR...) generates an expected tryptic peptide at m/z = 2109.9 ([M+H]⁺). Mature ApoA1 sequence generates a predicted N-terminal tryptic peptide (DEPPQSPWDR...) of m/z = 1226.5 ([M+H]⁺). AIM = Apoptosis inhibitor expressed by Macrophages (alternate name for CD5L).

ISSN: 1471-2407