Figure 4
From: Role of KCNMA1gene in breast cancer invasion and metastasis to brain
Differential BK Ca channel activity measured by membrane potential in breast cancer cell lines. The BKCa channel activity was measured in the presence of its activator NS-004 with and without siRNA for KCNMA1 gene. Pretreatment of breast cancer cell lines with siRNA significantly (*p < 0.046; **p < 0.001) inhibited the BKCa channel activity following addition of NS-004. Note: Decreased drop in fluorescence (indicator of BKCa channel opening/activity) of siRNA + NS-004- treated cells compared to NS-004 treated cells demonstrates that BKCa channel activity was inhibited by addition of siRNA to cells, as fluorescence quenching is an indirect measure of K+ ion-mediated hyperpolarization (hyperpolarization is caused by efflux of K+ through K+ channels), shown in the Y-axis. Bars represent the mean and standard error of the mean obtained from three different experiments in triplicate (n = 9).