Figure 2

Analysis of KCNMA1 gene expression by RT-PCR. Semiquantitative RT-PCR analyses of human primary breast cancer (n = 6), metastatic breast cancer {n = 6, sample 1 (metastasized to ovary), 2 and 3 (metastasized to liver), 4–6 (metastasized to lymph node)}, metastasized to brain (n = 4) and breast cancer cell lines reveals differential expression of KCNMA1 gene expression (α-subunit, 250 bp). GAPDH was used as the loading control. NB: Normal breast tissue, MCF-10A (normal breast cell line), MCF-7 (non mets), 231: MDA-MB-231 (Mets) and 361: MDA-MB-361 (Brain Mets). Note: Only one of six primary breast cancer tissues showed detectable amount of KCNMA1 RNA (see arrow).