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Figure 1 | BMC Cancer

Figure 1

From: Establishment and characterization of pleomorphic adenoma cell systems: an in-vitro demonstration of carcinomas arising secondarily from adenomas in the salivary gland

Figure 1

Histopathology of pleomorphic adenoma from which cells were isolated (A, HE stain, × 40; B, HE stain, × 200) and phase-contrast microscopy of pleomorphic adenoma cells (C, primary culture, ×100; D, colony with bizarre cells, ×135; E, established cell system SM-AP3, × 150; F, SM-AP5, × 150). The surgical specimen showed typical features of benign pleomorphic adenoma (A), containing only a small number of atypical tumor cells (B). Cells in the primary culture were mainly spindle in shape mixed with fewer amounts of polygonal cells (C). In the fourth passage, aggregates of polygonal and bizarre epithelioid cells appeared in the background of spindle-shaped cells (D). From the following passage, five clones were successfully grown and isolated. They were classified into two groups according to their cell shapes. One was a polygonal shape represented by SM-AP3 (E), and the other was a short spindle shape one as shown by SM-AP5 (F).

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BMC Cancer

ISSN: 1471-2407

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