Identification of proteins involved in neural progenitor cell targeting of gliomas

BMC Cancer

Table 2 Expression profiles of selected genes and proteins from microarray analysis, RT-PCR, immunocytochemistry and western blot analysis.

GENE CELL	N25	N32	N29	RN33B	ST14A	HIB5	ANALYSIS
Follistatin	+	-	+/-	-	+++	+++	Microarray
	+	-	-	-	+++	++	RT-PCR
	+	+/-	-	-	+++	+++	Protein exp
GAS6 (growth arrest specific protein 6)	+/-	+/-	+/-	+/-	+++	+++	Microarray
	+/-	+/-	+/-	-	+++	++	RT-PCR
	-	-	-	+/-	++	++	Protein exp
TAPA1/CD81	+	missing	++	+	+++	+++	Microarray
	+	+++	+	+/-	+++	++	RT-PCR
	++	+++	+++	-	++	+++	Protein exp
AXL	missing	missing	missing	missing	missing	missing	Microarray
	++	+	++	+	+++	++	RT-PCR
	+++	++	+++	-	++	+++	Protein exp
GRO1/CXCL1 (chemokine (C-X-C motif) ligand 1)	+++	++	++	++	+	+	Microarray
	++	+++	+	-	++	++	RT-PCR
	-	+++	++	-	-	-	Protein exp
TPT1 (tumor protein, translationally controlled 1)	++	+	++	+/-	+++	+++	Microarray
	+++	++	+	+/-	++	+	RT-PCR
TRIM23/Arfd1 (tripartite motif-containing 23)	+	+	+	+	+++	+++	Microarray
	+	+	+	++	+++	++	RT-PCR
CD82/Kai1	++	++	+++	+	+++	+++	Microarray
	+++	++	++	+	+++	+++	RT-PCR
Camk2 (Ca/calmodulin dependent protein kinase typeII)	++	+	+	+	+++	++	Microarray
	++	+++	+	+	++	++	RT-PCR
Eef1a (Elongation factor 1 alpha)	++	+	+	+	+++	+++	Microarray
	+++	+	+	+	++	++	RT-PCR

The order of the scores in the table is 1) microarray, 2) RT-PCR, 3) protein analysis. The selected and compared genes were: Growth arrest specific 6 (gas6), follistatin (fst), AXL receptor tyrosine kinase (missing on the array), translationally-controlled 1 (TPT1), Kai (CD82), CD81 (Tapa-1), ADP-ribosylation factor domain protein 1 (Arfd1), Calcium/calmodulin-dependent protein kinase II (Camk2), growth regulated protein precursor CXCL1 and Eukaryotic translation elongation factor 1 alpha 1 (Eefa1). The levels of expression are compared within each method of measurement used and expressed within each group as +++ for the strongest expression measured. ++ was set for values ranging from 45–70% of the highest score (+) for 15–44% of the highest score, (+/-) for 1–14% of the highest score and (-) for no expression detected.

ISSN: 1471-2407