Figure 4

RAI3 Immunohistochemistry on TMA derived from normal and cancerous breast tissue. Immunohistochemical expression analysis of RAI3 in normal breast tissue as well as non-invasive and invasive breast tumours using a tissue microarray. (A, B) In normal breast epithelial cells RAI3 expression (IRS = 8) was often weaker than in invasive breast carcinoma cells. (C, D) In ductal carcinoma in situ RAI3 expression (IRS = 3) was often less intense compared to invasive breast carcinoma. (E, F) In invasive breast carcinomas (here: ductal type) RAI3 was often expressed more abundantly in the cytoplasm and in the cell membrane (example shows staining with an IRS = 12) than in either ductal carcinoma in situ or normal breast tissue. (G, H) In tubular breast carcinomas, RAI3 expression was less abundant (IRS = 3) than in most invasive ductal breast carcinomas. (I, J) In mucinous breast carcinomas, RAI3 expression was also less intense (IRS = 1) in comparison to most invasive ductal breast cancer cells. Magnifications: A, C, E, G, I: 40×; B, D, F, H, J: 400×.