Figure 7

Assessment of the therapeutic window of compound 1 on Human normal astrocytes and on rat cortical neurons vs U87MG and DBTRG-05-MG. The cells were treated for three days with increasing concentrations of the compound 1. Cell viability was assessed using MTT. From the overlay of the curves (panel a) we assessed the therapeutic window of compound 1 by comparing cell viability of increasing concentrations of the compound (from 10 nM to 20 μM) on human normal astrocytes (green curve) versus GBM cell lines U87MG (black curve) and DBTRG-05-MG (red curve). The neurotoxicity was assessed testing compound 1 on rat cortical neurons proliferation (panel b). In panel c the same experiment was reported as bar graph to underline the statistical differences at each concentration point. U87MG (gray bars) and DBTRG-05-MG (light gray bars) cells started to be statistically different from 500 nM and 1 μM respectively when compared to normal human astrocytes (black bars) and rat pure cortical neurons (white bars) where compound 1 was un-effective.