Figure 5

DCA-Induced PARP cleavage and DNA fragmentation is Caspase Dependent. SKGT4 cells were treated with 50 μM Z-VAD-FMK or 50 μM Z-DEVD-FMK for 1 hr prior to the addition of 400 μM DCA for 6 hr. Whole cell lysates were standardized to 50 μg as described in experimental procedures. PARP cleavage was assessed by immunoblotting with an anti-PARP antibody followed by anti-α-tubulin as loading control (A). DNA fragmentation was assessed by ELISA (B). Results are given as fold induction of DNA fragmentation relative to unstimulated cells. Mean ± SD. Results are representative of at least two independent experiments.