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Table 3 BLU, RASSF1A and MGMT genes hypermethylation in astrocytoma and neuroblastoma cell lines.

From: Detection of methylation in promoter sequences by melting curve analysis-based semiquantitative real time PCR

 

BLU

RASSF1A

MGMT

 

MSP

MCA-MSP

MCA-Meth

Seq

MSP

MCA-MSP

MCA-Meth

Seq

MSP

MCA-MSP

MCA-Meth

Seq

U87

U

 

U

0%

 

U+M

 

U+M

36%

 

M

 

UM

 

UM

GOS 3

M

M

M

100%

 

M

M

M

100%

 

U+M

U+M

U+M

78%

 

A 172

M

M

M

100%

M

M

U+M

M

100%

 

M

M

UM

  

MOG

U+M

M

U+M

53%

 

M

UM

UM

  

U+M

UM

UM

  

T98G

U

U

U

0%

 

M

M

M

67%

M

M

UM

M

61%

 

U118

U

 

U

0%

U

M

 

M

100%

 

M

 

M

98%

 

SIMA

U+M

M

M

76%

 

M

M

M

100%

 

U

U

U

0%

 

IMR 32

U+M

U

U

0%

 

M

M

M

98%

 

U+M

UM

M

86%

 

SK-N-MC

U

U

U

0%

 

M

M

M

100%

 

U+M

U

UM

  

MC-IXC

U

U

U

0%

 

M

M

M

100%

 

U

U

U

0%

 
  1. U, unmethylated peak or band; M, methylated peak or band; U+M, sample producing both methylated and unmethylated peaks or bands; MU, sample producing only one peak at an intermediate temperature; blank spaces: not determined analyses. The percentage of fully methylated DNA molecules present in the samples (semiquantitative methylation study) is shown for the MCA-Meth in the M and U+M cases. On the contrary, no percentage appears in the the UM cases, as the UM corresponds to partially methylated DNA molecules, that can not be semiquantitatively studied according to the curve obtained with different proportions of fully methylated DNA.
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BMC Cancer

ISSN: 1471-2407

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