Figure 4

Determination of the percentage of fully methylated MGMT by MCA-Meth. a) The graph shows the melting peaks for the different percentages of methylated and unmethylated DNA. The increase and the decrease in the height of the specific peaks in the different percentages can be observed. Red: 0% methylated DNA. Green: 100% methylated DNA. Purple: 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80% and 90% methylated DNA. b) Graph representing the linear relationship between the % of methylated DNA and the height of the methylated peak in the melting curve of the MGMT gene. As the % of methylated DNA increases (0%, 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100%) the peak appears higher. Measuring the height of the peak of each sample, and interpolating the data in the graph, we can obtain an approximate % of fully methylated DNA present in each sample.