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Figure 1 | BMC Cancer

Figure 1

From: Cigarette smoke induces endoplasmic reticulum stress and the unfolded protein response in normal and malignant human lung cells

Figure 1

Induction of eIF2α phosphorylation in NHBE cells by cigarette smoke and thapsigargin. Panel A: NHBE cells were exposed to air (M = mock treatment) or 2R4F cigarette smoke with 35 cc puffs diluted in 250 cc air (CS = smoke treatment) for 20 minutes. Cells were then placed in fresh medium and returned to the incubator for the time periods specified. Western blots of whole cell lysates were probed with antibodies to phosphorylated eIF2α, eIF2α, and GAPDH as a loading control. C = untreated control. Panel B: NHBE cells were treated with either 1 uM thapsigargin in DMSO (T), or DMSO (VC = vehicle control) for the times specified. Western blots of whole cell lysates were probed with antibodies to phosphorylated eIF2α, eIF2α, and GAPDH. C = untreated control.

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BMC Cancer

ISSN: 1471-2407

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