Table 2 Performance of the qPCR. Standard curve results are summarized and the amplification efficiency calculated.
From: Identification of genes for normalization of real-time RT-PCR data in breast carcinomas
Water | gDNA | |||||
|---|---|---|---|---|---|---|
Dilutant | ||||||
Genes | Slope | R2 | Amplification efficiency | Slope | R2 | Amplification efficiency |
PUM1 | -3.49 | 0.998 | 1.9 | -3.47 | 0.998 | 1.9 |
TBP | -3.36 | 0.988 | 2.0 | -3.27 | 0.998 | 2.0 |
RPLP0 | -3.56 | 0.999 | 1.9 | -5.70 | 0.924 | 1.5* |
ACTB | -3.52 | 0.999 | 1.9 | -4.91 | 0.940 | 1.6* |
GAPDH | -3.27 | 0.999 | 2.0 | |||
B2M | -3.45 | 0.998 | 2.0 | |||
ABL | -3.41 | 0.999 | 2.0 | |||
GUS-B | -3.33 | 0.998 | 2.0 | |||