Figure 7

DRSP and NES promote T47-D breast cancer cell horizontal migration. (A-B) Cells were treated with DRSP (100 nM) or NES (1 nM) for 48 h, in the presence or absence of ORG 31710 (ORG – 1 μM), of PD98059 (PD – 5 μM), of wortmannin (WM – 30 nM), of PTX (100 ng/mL) or of Y-27632 (Y – 10 μM). T47-D cells were scraped out of the cell culture dish and the extent of migration of the remaining cells was assayed in non-proliferating cells in the presence of Ara-C. Representative images of cell migration are shown. (C-D) Cell migration distances were measured and values are presented as % of control. * = P < 0.05 vs. control; # = P < 0.05 vs. DRSP or NES; The experiments were performed in triplicates and data representing the migration distance of cells from the starting line are expressed as mean ± SD.