Table 1 EGFR HRM and sequencing primer sequences
Exon | Primer name | Sequence | Amplicon size |
|---|---|---|---|
Sequencing# | Â | Â | Â |
18 | EGFR18_m13_F | 5'-CATGGTGAGGGCTGAGGTGA-3' | 233 bp |
| Â | EGFR18_m13_R | 5'-CCCCACCAGACCATGAGAGG-3' | Â |
19 | EGFR19_m13_F | 5'-GTGCATCGCTGGTAACATCCA-3' | 294 bp |
| Â | EGFR19_m13_R | 5'-GGAGATGAGCAGGGTCTAGAGCA-3' | Â |
20 | EGFR20_m13_F | 5'-CGCATTCATGCGTCTTCACC-3' | 356 bp |
| Â | EGFR20_m13_R | 5'-CTATCCCAGGAGCGCAGACC-3' | Â |
21 | EGFR21_m13_F | 5'-TGGCATGAACATGACCCTGAA-3' | 295 bp |
| Â | EGFR21_m13_R | 5'-CAGCCTGGTCCCTGGTGTC-3' | Â |
HRM | Â | Â | Â |
18 | EGFR_ex18_F | 5'-CATGGTGAGGGCTGAGGTGA-3' | 199 bp |
| Â | EGFR_ex18_R | 5'-CCAGAGG(A*)CTGTGCCAGGGAC-3' | Â |
19 | EGFR_ex19_F | 5'-GTGCATCGCTGGTAACATCCA-3' | 250 bp |
| Â | EGFR_ex19_R | 5'-AAAGGTGGGCCTGAGGTTCA-3' | Â |
20a | EGFR_ex20a_F | 5'-AAGCCACACTGACGTGCCTCT-3' | 121 bp |
| Â | EGFR_ex20a_R | 5'-GCGTGATGAG(G*)TGCACGGT-3' | Â |
20b | EGFR_ex20b_F | 5'-CCTCCACCGTGCA(C*)CTCATC-3' | 146 bp |
| Â | EGFR_ex20b_R | 5'-CCCGTATCTCCCTTCCCTGA-3' | Â |
21 | EGFR_ex21_F | 5'-CCTCACAGCAGGGTCTTCTCTG-3' | 210 bp |
| Â | EGFR_ex21_R | 5'-TGGCTGACCTAAAGCCACCTC-3' | Â |