Table 1 Primers and restriction enzymes used for genotyping analyses
From: Case-only study of interactions between metabolic enzymes and smoking in colorectal cancer
Gene | Primers(5'-3'; F = forward, R = reverse) | Restriction enzyme |
|---|---|---|
CYP1A2 C734A | F: CCC AGA AGT GGA AAC TGA GA | ApaI |
| Â | R: GGG TTG AGA TGG AGA CAT TC | Â |
CYP1B1 C1294G | P1: TGT CCT GGC CTT CCT TTA TGA | - |
| Â | P2: GCC TCT TGC TTC TTA TTG GCA | - |
| Â | S1: CCG GGT TAG GCC ACT TGA C | - |
| Â | S2: GTC TGT GAA TCA TGA CCG AC | - |
CYP2E1 C-1019T | F: CCA GTC GAG TCT ACA TTG TCA | RsaI |
| Â | R: TTC ATT CTG TCT TCT AAC TGG | Â |
GSTM1 | F: TTC TGG ATT GTA GCA GAT CA | - |
| Â | R: CGC CAT CTT GTG CTA CAT TGC | - |
GSTT1 | F: GCC CTG GCT AGT TGC TGA AG | - |
| Â | R: GCA TCT GAT TTG GGG ACC ACA | - |
β-Globin | F: CAA CTT CAT CCA CGT TCA CC | - |
| Â | R: GAA GAA CCA AGG ACA GGT AC | - |
SULT1A1 G638A | F: GGT TGA GGA GTT GGC TCT GC | HhaI |
| Â | R: ATG AAC TCC TGG GGG ACG GT | Â |