Figure 8From: Elevated expression of VEGFR-3 in lymphatic endothelial cells from lymphangiomas Isolation and characterization of LECs from lymphangiomas. Lymphendothelial cells (LECs) from lymphangioma explants isolated by „cell sweeping” were photographed under phase contrast microscopy. a) The cells from patient-A show the typical cobblestone morphology and are contact inhibited (passage 7, magnification, × 40.). Expression of CD31/PECAM-1 together with Prox1 in LECs (b). Membranous localization of CD31 and nuclear localization of Prox1 was detected by immunocytology. Magnification, × 200. c) Same specimen as in b) showing CD31 expression and nuclear counter-staining with DAPI. Magnification, × 200. d) Podoplanin staining. Nuclei were counter-stained with DAPI. One representative staining out of several independent experiments is shown.Back to article page