Figure 2

CD40L L cells inhibit the cytostatic effects of anticancer drugs. Cells were incubated with (A) ETO (7,5 μg/ml), (B) CDDP (7,5 μg/ml), (C) VIN (50 nM), (D) TAX (50 nM), (E) DOX (0.5 μg/mL), in the presence or absence of irradiated CD40L L cells or irradiated CDw32 L cells (in panel E), then washed and cultured without drugs but otherwise in the same conditions during 24 additional hours before measurement of [³H] TdR incorporation. *: [³H] TdR incorporation was significantly increased (Student t test, p < 0.05) upon co-culture with irradiated CD40L L cells as compared to no L cells, or L cells expressing CDw32 (E). These results are the mean and SE of triplicate and these results are representative of 4 different experiments.