Figure 2

AC stimulation enhance doxorubicin efficacy. (a) Determination of doxorubicin toxicity by AK. As doxorubicin concentration increased, cells underwent significant detrimental changes. These were further increased by either MDR1 blockade or electrical stimulation. The asterisk refers to a statistical significant difference (n = 10; p = 0.028 by ANOVA) with non-stimulated cultures while the positive sign refers to statically significant differences (n = 10; p = 0.036) with cultures exposed to XR9576. Note that AC stimulation or exposure to XR9576 does not cause any cytotoxic effect in comparison to non-treated (control) cultures. (b) Quantitative analysis of the experiment shown in figure (1), including adult response for the chemotherapeutic agent doxorubicin. Note that electrical stimulation achieved an equal or greater effect than blockade of MDR1 by XR9576. The asterisk indicates a significant difference (n = 10; p = 0.023 by ANOVA) from controls whereas the sign plus symbolizes significant difference (n = 10; p = 0.031) from XR-treated cultures. Data points were fitted by the following sigmoid: Equation: y = A2 + (A1-A2)/(1 + (x/x0)^)