Figure 1From: Upregulation of meiosis-specific genes in lymphoma cell lines following genotoxic insult and induction of mitotic catastrophePost transcriptional accumulation of p39mos protein in response to 10 Gy irradiation. (A) Transcription of MOS from Namalwa, WI-L2-NS or TK6 cells on different days after irradiation. Messenger RNA was first isolated from samples and then reverse transcribed to yield cDNA for semi-quantitative PCR. Primers for GAPDH were used to verify integrity and quantity of cDNA. Mos expression was observed in all 3 cell lines with minor differences seen during the five days after irradiation; (B) upper panel: in comparison to non-treated control (NT), accumulation of p39mos was detected by Western blotting on days 1 to 4 afterirradiation, with maximum expression observed on days 2–3 in Namalwa, and days 1 and 2 for WI-L2-NS cells. In TK6 cells, p39mos was not detectable after irradiation. Lower panel: for loading control PVDF membranes were re-blotted with mouse monoclonal anti-b-actin antibodies.Back to article page