Figure 2
Effect of plasminogen in tumor metastasis. Metastatic brain tumors were established by injecting 106 B16F10 cells in the left internal carotid artery of wild type wild type C57Bl (wt), plasminogen deficient (plg-/-) and wild type mice treated with EACA (EACA). Fourteen days after tumor cell injection the mice were sacrificed the brains removed fixed and stained with hematoxylin eosin. The areas of the tumors at 200 μm intervals were measured and the total volume of the tumor determined. Note the reduced tumor size in mice treated with EACA and plg-/- mice compared to wild type mice. B) Survival graph of 12 wild type mice (wt), 11 plg -/- mice (plg-/-) and 7 wild type mice treated with EACA (EACA) injected with 106 B16F10 cells. Note the increased survival of plg -/- and wild type mice treated with EACA compared to untreated wild type mice (p < 0.03)