Figure 1From: Quantitative real-time RT-PCR validation of differential mRNA expression of SPARC, FADD, Fascin, COL7A1, CK4, TGM3, ECM1, PPL and EVPLin esophageal squamous cell carcinomaThe relative standard curve using the TGM3 gene, primers, and probes amplified with 100, 10, 1, 0.1 and 0.01 ng of total RNA. Each sample was run in triplicate (red square). The average CT values (y-axis) are plotted against the logarithm of the input amount of RNA (x-axis) added to each sample. PCR efficiency (E) = (10-1/slop - 1). It shows a linear relationship between RNA concentration and the CT value of reverse transcription real-time PCR reaction for TGM3.Back to article page