Figure 1
From: Reduced repair of 8-hydroxyguanine in the human breast cancer cell line, HCC1937
Incision of 8-OH-Gua by AG11134 and HCC1937nuclear extracts. 5' end-labeled duplex oligonucleotides containing single 8-OH-Gua were incubated at 37°C with 50 μg of nuclear extracts and aliquots removed at specified intervals as shown in the figure. The Fpg reactions were performed for 30 min. The reactions were stopped by the addition of 0.25% SDS and heating at 90°C for 10 min. The DNAs were purified by phenol/chloroform/isoamyl alcohol extraction method and then separated by electrophoresis through 20% denaturing gels. The Panels are: A, 8-OH-Gua incision by AG11134 nuclear extracts; B, 8-OH-Gua incision by HCC1937 nuclear extracts; C, 8-OH-Gua incision by MCF-7 nuclear extracts and D, quantified incision of 8-OH-Gua by AG11134, MCF-7 and HCC1937 nuclear extracts. The results are representative of three independent experiments and the error bars represent SEM.