Figure 5

Effects of PD0166285 on expression related protein of cell cycle regulators. A: Lysates (30 μg) were prepared from cells after treatment with 0.5 μM PD0166285 for the indicated period (0 h, 2 h and, 4 h) were subjected to immunoblot analysis with anti-cyclin D, anti-cyclin E, anti-p16, anti-p21, and anti-p27 antibodies. B: Cell lysates after treatment with 0.5 μM PD0166285 for long periods treatment; (0 h, 4 h, and 24 h) were subjected to an immunoblot analysis with Anti-Rb antibody and cell cycle suppression directly molecule p21 and p27.