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Figure 3 | BMC Cancer

Figure 3

From: Cell cycle regulation by the Wee1 Inhibitor PD0166285, Pyrido [2,3-d] pyimidine, in the B16 mouse melanoma cell line

Figure 3

Effects of PD0166285 on the G2 phase. A: Immunoblot analysis of anti-cdc2, anti-phosphorylated cdc2-tyr15, and Wee1 was performed in lysates of with 0.5 μM PD0166285 treated for 0 h, 2 h, or 4 h. B: Subcellular location of cyclin B was observed. After 4 h of treatment with 0.5 μM PD0166285, cells were fixed and doubly stained for immunofluorescence with PI (red) and anti-cyclin B1 (green). Shown are typical merged images obtained by confocal microscopy. Bar: 10 μm.

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BMC Cancer

ISSN: 1471-2407

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