Figure 1
From: p150 ADAR1 isoform involved in maintenance of HeLa cell proliferation
A. Effect of Dox induction on expression of mRNA for p150 isoform of ADAR1 in HeLa-Rz cells. B. Time-dependent knockdown of ADAR1 p150 gene in HeLa-Rz cells by Dox induction. RNA was isolated from HeLa-Rz cells treated with 2 μg/mL of Dox or left untreated and then was analyzed by RT-PCR. PCRs were carried out using primers that flank exons 1–2 of human ADAR1 mRNA (representing p150 isoform) or β-actin plus and minus primers, respectively. PCR products were analyzed by gel electrophoresis after the indicated number of amplification cycles.