Figure 2

Cytoskeleton modeling in Rat-1 cells expressing t(9;22) translocation products. A. The provirus used for transduction of the cell line. Expression of the transgenes is driven by the 5'-LTR (long-term repeat), whereas the EGFP-reporter gene is under the control of the CMV promoter (CMV-P). B. Provirus used for the expression of the transgenes. The sizes are not to scale. C. Expression of the transgenes in Rat-1 cells was controlled by Western blot analysis using α-ABL and α-BCR antibodies as indicated. D. Rat-1 cells expressing the indicated transgenes stained with phalloidin to reveal the actin cytoskeleton. mock – empty vector infected cells as control. Control – mock infected Rat-1 cells; Rac* – V12Rac (constitutively activated form of Rac), cdc42* – V12cdc42 (constitutively activated form of cdc42), Rho* – V14Rho (constitutively activated form of Rho). The bottom panel shows the Rat-1 cells co-expressing BCR/ABL together with BCR or its mutants.