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Figure 1 | BMC Cancer

Figure 1

From: Histone deacetylase inhibitors strongly sensitise neuroblastoma cells to TRAIL-induced apoptosis by a caspases-dependent increase of the pro- to anti-apoptotic proteins ratio

Figure 1

Subtoxic doses of NaB, SAHA and TSA sensitise NB cells to TRAIL-induced cell death. A Sensitisation is dependent on the concentration of TRAIL. SH-EP and SK-N-AS cells were treated with the indicated amount of TRAIL and NaB, SAHA or TSA for 48 h. Cell death was measured by MTS/PMS cell proliferation assays. Mean values of three or two independent experiments are shown for SH-EP or SK-N-AS cells, respectively. B Subtoxic doses of NaB, SAHA and TSA sensitise NB cells to TRAIL-induced cell death. Sub-G1 apoptotic cells were detected by the propidium staining method after stimulation for 16 h or 48 h with 1 mM of NaB, 2.5 μM of SAHA or 0.5 μM of TSA in absence or in presence of TRAIL (25 ng/ml) and M2 (125 ng/ml). C Subtoxic doses of NaB, SAHA and TSA sensitise NB cells to TRAIL-induced cell death. Sensitisation is dependent on the concentration of HDACIs. SH-EP cells were treated with TRAIL 50 ng/ml and M2 (250 ng/ml) and SK-N-AS cells with TRAIL 100 ng/ml and M2 (500 ng/ml), and indicated amount of NaB, SAHA or TSA for 48 h.

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BMC Cancer

ISSN: 1471-2407

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