Table 1 Primers used in the PCR study and medians (range) of mRNA expression of hBDs in patients with basal cell carcinoma and healthy controls.

hBD-1

F 5'-AGATGGCCTCAGGTGGTAACTTT-3'; R 5'-GGGCAGGCAGAATAGAGACATT-3'

hBD-2

F 5'-TGATGCCTCTTCCAGGTGTTT-3'; R 5'- GGATGACATATGGCTCCACTCTT-3'

hBD-3

F 5'-TCCATTATCTTCTGTTTGCTTTGC-3'; R 5'-TTCTGTAATGTGTTTATGATTCCTCCAT-3'

GAPDH

F 5'-CCTCAACTACATGGTTTACATGTTCC-3'; R 5'-ATGGGATTTCCATTGATGACAAG-3'

Medians and range of mRNA expression

lesional

non-lesional

healthy controls

hBD-1

0.154 (0.002 – 0.634)

0.389 (0.111 – 0.941)

0.431 (0.161 – 0.981)

hBD-2

0.053 (0.000 – 0.974)

0.008 (0.001 – 0.162)

0.004 (0.000 – 0.178)

hBD-3

0.057 (0.002 – 0.915)

0.048 (0.006 – 0.263)

0.041 (0.004 – 0.541)