Figure 6

Increased androgen receptor expression does not block the inhibitory effect of C/EBPα on PSA promoter activity. LNCaP cells were co-transfected with 0, 10, 100, and 200 ng of androgen receptor cDNA in the presence (dark bars) or absence (open bars) of 100 ng cDNA for C/EBPα and in the presence of luciferase reporter construct driven by either the promoter/enhancer (upper panel) or PSA proximal promoter (lower panel). DHT was added at 50 nM and after 36 hrs the luciferase activity was measured. The results expressed as relative light units are the means ± standard deviation of 3 separate experiments.