Figure 5

Dose-dependent inhibition by C/EBPα of the activity of PSA promoter/enhancer. Prostate cancer cells, ALVA101, PC3, and LNCaP cells were transiently transfected either with 0, 20, or 100 ng C/EBPα in the presence (dark bars) or absence (open bars) of DHT. Fifty ng of the androgen receptor expression vector (also the kind gift of Dr. Stephen P. Balk) was co-transfected into the AR negative cell lines, ALVA101 and PC3. The results are expressed as in Figure 4A with the double star representing a p-value < 0.01, a single star, p-value < 0.05, and the open and dark stars representing the same comparisons as in Figure 4A.