Table 2 List of genes identified in the microarray analysis between HeLa Tet-off cells harboring pTIG-TSPY and those harboring the vector pTIG alone

TSPY1

NM_003308

26.5

Testis specific protein, Y-linked, stably transfected gene in the cells

MAP2

NM_002374

3.41

Microtubule-associated protein 2, neurogenesis and tumorigenesis

RGC32

NM_014059

2.56

Response gene to complement 32, deregulation of cell cycle required for tumor cell growth

PDGFC

NM_016205

2.32

Platelet derived growth factor C, a mitogenic factor

MAP1B

NM_005909

1.93

Microtubule-associated protein 1B, structural protein, neural development, predictor for breast cancer

EFEMP1

NM_004105

1.79

EGF-containing fibulin-like extracellular matrix protein 1, growth factor

G1P2

NM_005101

1.72

α-interferon inducible protein, IFI-15K, facilitates viral growth

LUM

NM_002345

1.62

Lumican, epithelial cell migration and tissue repair

NANOS1

NM_199461

1.57

Human nanos homolog (of Drosophila), germ stem cell development expressed in spermatogonia and spermatocytes in human testis

WNT5A

NM_003392

1.55

Wingless-type MMTV integration site family member 5A, Wnt gene family, implicated in oncogenesis and embryogenesis

CD24

NM_013230

1.55

Small cell lung carcinoma cluster 4 antigen, high expression in SCLC

TMTC1

NM_175861

1.53

Transmembrane and tetratricopeptide repeats containing 1, multi-functional, possibly involved in regulation of cell cycle and/or mitosis.

CCND2

NM_001759

1.46

Cyclin D2 complexes with CDK4 or CDK6, G1/S transition, amplified and over-expresses in numerous types of tumors

RAP1A

NM_002884

1.44

RAS oncogene family member

EGFR

NM_005228

1.41

Epidermal growth factor receptor (ERBB oncogene), cell proliferation, amplified and expresses at high levels in many tumors

ANKRD15

NM_015158

1.38

Ankyrin repeat domain 15, tumor cell growth in renal cell carcinoma

GSPT1

NM_002094

1.32

G1 to S phase transition 1, an inhibitor of apoptosis

EP300

NM_001429

1.26

E1A binding protein p300, a co-factor for hypoxia inducible factor 1A

CDKN2B

NM_004936

Cyclin-dependent kinase inhibitor 2B (p15), binds to and prevents CDK4/CDK6 activation and G1/S progression, tumor suppressor

CUL1

NM_003592

Cullin 1, involves in deneddylation and modulates G1/S transition

TGFB3

NM_003239

Transforming growth factor, b3, suppresses tumor formation and blocks cell cycle progression

PTN

NM_002825

Pleiotrophin, neurite growth promoting factor 1, down-regulated in breast cancer

TIMP1

NM_003254

Tissue inhibitor of metalloproteinase 1, suppresses tumor growth and metastasis

DUSP5

NM_004419

Dual specificity phosphatase 5, inactivates kinases by dephosphorylation, and negatively regulates MAP kinases

SPARC

NM_003118

Secreted protein, acidc cysteine-rich (osteonectin), loss of expression in lung cancers, regulates cell proliferation

CLU

NM_001831

Clusterin, proapoptotic in colon cancer, down-regulated in CaP

IGFBP3

NM_000598

Insulin-like growth factor binding protein 3, pro-apoptotic, its inactivation or repression is essential for growth of various tumors